Just had a great couple of days at the 15th Sun Protection Conference held at the Royal College of Physicians in London on the 25th and 26th November 2021. Great selection of speakers talking about a wide range of aspects of sun protection and what the future holds for it. There are certainly challenges ahead for the industry in the next few years, which worryingly seems to be driven by a lack of understanding of what the ingredients in sunscreens are, how they work, and their interaction with us and the environment. Misreporting and/or misunderstanding of science by influencers and the media has done its usual job of stoking fear in the consumer, and sowed seeds of doubt with regards to their safety and benefits. On a more positive note, there was strong science presented on the benefits of sun protection, and the work going into the development of better products and consumer education.
The conference included talks from a range of industry and academic experts as well as physicians. I gave a talk on UV imaging and microscopy, and covered areas such as how to take photographs in the UV, the behavior of melanin at different wavelengths, the importance of knowing the limitations of the methods you use (an area which is far too often overlooked), my UV microscope build and what sunscreens look like when imaged with it and imaging in UVC.
Looking around the conference it made me realise that we need to get new people to give talks and present their work. Don’t get me wrong, it is great to hear from experts who have worked in this field for many years, but we also need new perspectives. It can be very daunting to get up and speak in these types of events, but remember that everyone is there because they have an interest in this area and are passionate about it. With sun protection we are also there to save lives. I strongly recommend anyone who has work they want to share with the world to reach out to conference organizers and see whether there is the opportunity to present their work.
This even turned out to be even more special for me, as I was given the award for the conference for my talk, by the International Journal of Cosmetic Science. As a result I have the opportunity to write and publish a paper covering the work from my talk as an article in the journal (assuming it passes peer review) as an Open Access article. Normally the author has to pay to make the article Open Access, but the award covers the cost for this. This means that the article will be open to anyone to read, rather than people who have subscribed to the journal or who are in a position to pay for the article.
A big thank you to Dr Jack Ferguson and the team at Summit Events for organizing a great event, and I’m already looking forward to the next one in 2 years time. Hopefully we’ll see more of you there.
Some projects I do are directly related to a specific industry related problem. Others are more of the ‘I wonder if if is possible?’ type. Todays post is one of the latter ones. Lenses specifically designed for UV photography such as the Rayfact 105mm UV, Asahi 85mm Ultra Achromatic Takumar, and 60mm Coastal Optics f4, are all manual lenses. This is fine for most applications, but something I have always wondered about is whether it would be possible to make an autofocus UV lens? The end result was compact lens using a Nikon autofocus lens body and a 79mm Thorlabs aspheric UV fused silica element, and this is how I went about it.
A few weeks ago, a post on the Ultraviolet Photography forum described how someone had taken a Canon EOS 50mm f1.8 lens apart and installed a 75mm plano convex lens in place. The result was an autofocus setup able to be used on Canon cameras. Readily this spurred me on, and I decided to try and do one myself. I wanted to use a Nikon lens body, as they have mechanical aperture dials, and I can use them on my Canon, Nikon and Sony cameras with the adapters I have. I sourced a Nikon 50mm f1.8 AF lens from a charity shop and set about taking it to bits to remove the original lens elements. This was relatively simple, and I managed to get the glass elements out without damaging them.
This is the lens design, again from Ken’s page linked above. I’ve added in 1-6 in red for the elements.
Lens elements removed, I got the following.
As these came out without damage, I thought it’d be nice to measure their transmission and see what that looked like. This is what I got.
Note, I’ve included a ‘Theoretical’ line in the graph as well, which would be all the elements combined. Unfortunately I was so excited when it arrived that I took it apart before measuring the transmission of the lens, so don’t have an ‘unmodified’ lens graph to compare against. Bad scientist…..
The cemented doublet seems to be the one restricting the transmission the most (as expected). What surprised me though was element 6 (the rear element). This is a plano convex lens with a focal length of about 65mm, and it has a really deep UV transmission unlike the 3 single elements at the front of the lens. I wonder what glass they used for that?
Once the glass was out, I was left with and empty lens body, so I decided to glue in a Thorlabs SM1 tube to the body to act as a mounting point for lenses. This was pretty much a perfect fit, and required very little work to install.
I decided I wanted to use my Thorlabs 79mm aspheric UV fused silica lens for this build, and with it screwed in place, in visible light, the lens was able to focus from infinity down to just over a meter. Even better, the autofocus function on my Nikon d800 worked, and the lens did indeed autofocus properly. I can adjust the aperture from the camera, or mechanically on the lens, and I can focus either manually or from the camera. Yay.
Now it should be noted that wide open it is certainly not sharp at the edges of the image, which is to be expected. Also, wide open the contrast is soft, and very prone to flare, so a lens hood is essential.
Does it work in the UV? Interestingly, in the UV when using a Baader U filter, the lens no longer focuses to infinity. In hindsight that isn’t a huge surprise, and I’d expect it to be more of an issue the further into the UV I go with it. It does however still focus with more close up subjects. This was a picture of the trunks of cherry trees in my garden, taken in the UV with the Baader U filter and Nikon d800 camera.
The lens will certainly autofocus in the UV (if there is sufficient UV for it to do so). It’ll be interesting to see if this still works even as far into the UV as 254nm.
The good thing about using the Thorlabs SM1 tube means that mounting other lenses becomes quite simple if I want to try them out. For instance this is a rather more chunky OptoSigma80mm excimer lens which is triplet construction. This does fit (as shown below), and the focus system still works despite it being much heavier. In theory this should offer better correction than the single Thorlabs aspheric lens, but that remains to be tested.
This was a fun little build, and the result is a very compact UV capable autofocus lens which I can use on a variety of camera bodies (although it will be autofocus only on my Nikons). Will it change how I do my imaging work? I doubt it, but I got to learn about how lenses work, and it showed that it was indeed possible to use existing camera lens bodies to carry new and interesting optical elements. I also got to learn a bit more about the optical properties of the glasses used in commercial camera lenses. Thanks for reading, and if you’d like to know more about this or any other aspect of my work, I can be reached here.
Some experiments are complex to setup but easy to do, others are in theory simple to attempt but in practice quite time consuming to carry out. This little experiment was not particularly hard to do, but took quite a lot of time to setup and get the images and process the data. Quite a neat result though, so I thought it was worth sharing.
This came about after a discussion on the Ultraviolet Photography forum, about the behaviour of the metal silver in the ultraviolet. Silver should have a strong dip in its reflection curve at around 320nm, as shown here, and it got me wondering whether I could image that with my UV camera setup. Not having loads of silver laying around the house I ordered a couple of sheets of edible silver leaf which is used in cooking, and mounted some on a piece of cardboard.
Imaging wise, I placed the sample in a box painted with Semple Black 3.0 paint to keep the reflections down. I also included a 99% Spectralon diffuse reflectance standard along with it. This was so that I could balance the exposures at different wavelengths and loo for differences in how the silver appeared. Camera was a monochrome converted Nikon d850 from MaxMax, lens was a 105mm UV Rayfact. Filters were a mix of Baader UV/IR for visible light, Thorlabs and Edmund Optics bandpass ones for 390nm to 300nm (along with a Hoya U-340 4mm for the 300nm image as the bandpass filter leaked a bit), and a 254nm bandpass filter from a forensics camera. Light source was a Hamamatsu LC8 200w xenon lamp which I used down to 300nm and a UVP 254nm filter lamp for 254nm. You can see now why it was time consuming.
The images were as follows.
The images did indeed show a very sharp drop in reflectance of the silver at around 320nm. Yay, the images back up the data from the reflectance graph. Good to know the edible silver leaf is indeed silver.
Including the Spectralon standard allowed me to compare the channel response from the silver with that of the Spectralon, and plotting that gave the following.
Plotting out the channel response does indeed show the strong dip in reflectance at 320nm and matched the literature data well down to 300nm. At 254nm though the match is not so good, and I am not 100% sure as to why that is. I believe that Spectralon reflectance starts to drop around 250nm, and I know from imaging at 254nm before that any organic material would be highly absorbing at that wavelength. As a result the 254nm image of Spectralon may be darker than it should be as my sample certainly could do with a clean. If this is darker than expected then it would make the silver seem more reflective than it is. It should also be stressed that at 254nm camera sensitivity is extremely low so of all the data points this is the one I am least confident in. More work needed on that from me to understand it better….
Why bother doing this you may ask, why use a camera in this way? Well, that lovely antique you are interested in buying that is supposedly silver, do you really know that it is? This type of technique can be used to check what materials actually are, and can sometimes be simpler to implement than x-ray methods and less damaging to the samples. However my main interest was curiosity – could I use a camera to see the behaviour of silver in the UV trying something to see what will happen. After all, a lot of research starts with a a curious scientist. Thanks for reading, and if you’d like to know more about this or my other work, you can reach me here.
Had a great time yesterday giving a talk to the Royal Photographic Society Imaging Science Group about imaging skin with ultraviolet, visible and infrared light.
Active for over 100 years, you can read more about the Imaging Science Group and their activities here, as well as finding out about their upcoming talks for October and November. My talk covered cross polarization, UV and IR photography of skin as well as applications of UV fluorescence in dermatology. The talk was recorded, and can be viewed on the RPS Imaging Science Group website, along with the other talk from the evening on UV reflected and fluorescence imaging of plants by my fellow speaker Adrian Davies, if you’d like to view it.
Thanks again to the folks of the Imaging Science Group for inviting me to talk to them.
Erythema (skin redness) is a vital marker for showing the effects of UV exposure on skin. Took the team through the range of methods and approaches for assessing and quantifying erythema, along with the watch-outs and considerations that are needed.
Great to have the opportunity to offer training to the team on such an interesting area.
As researching the photographic imaging process is part of my work, I am lucky enough to have access to a number of amazing lenses. Today I share with you the Nikon Macro Nikkor 65 mm and 12 cm lenses. These are not to be confused with Nikons usual macro lenses (actually Nikon calls these Micro Nikkors), the Macro Nikkors were developed for the Multiphot large format photomacrography and photomicrography system. Confused yet? If so don’t worry, basically they are M39 threaded, completely manual lenses, designed for close up work with a bellows. I wont share much about their background here, as there has been plenty written already about them (for example, see here).
Why my interest in them? Well, they are great macro lenses, with a bit of a reputation among photographers, and as always I am interested in learning more about the more unusual pieces of equipment. As always I’m also interested in whether or not lenses have the potential for use with UV imaging. I was recently fortunate enough to be able to get two of the four that were made – the 65mm and 12cm ones – along with an adapter to fit them to Nikon bayonet mount for mounting on a normal camera. Here’s how they look. First the 12cm.
And the 65mm one along with the adapter.
What’s their image quality like. As a quick test shot here’s a close up of a flower, taken with the 65mm one on bellows, using one of my Canon EOS 5DSR cameras and with ambient light in the room. This was pretty much the full frame, but reduced in resolution for sharing online.
And now a crop from the original of the image above, shown at the actual pixel resolution of the image.
Given this was done with ambient light rather than flash, so the exposure time was 4 seconds, I’m quite happy with the quality it produced.
How did they look for UV transmission? As always I test my lenses with my Ocean Insight FX spectrometer for transmission between 280nm and 420nm, and here’s how they performed.
Not the best for UV transmission, although the 12cm one has more reach than the 65mm one. Not hugely surprising, given the optical coatings they have, and their overall construction. C’est la vie, although they would still be potentially useful in the 360nm to 400nm range.
Bit a brief update today, but back to work now. This cutting edge science wont do itself you know. Thanks for reading and if you would like to know more about this or other aspects of my work, you can reach me here.
As discussed here, I was recently fortunate enough to be able to spend a few days evaluating a Phase One IQ4 Achromatic camera, courtesy of Teamwork Digital Ltd here in the UK. This really interested me as it is a medium format (53.4mm x 40.0mm) back side illuminated sensor with no Bayer filter layer. Given my work imaging outside of the visible spectrum, this had a lot of potential, so I wanted to see what it could do. I wont go through all specs of the camera here, but this is prestige piece of imaging equipment and for more information about it head to the Phase One website.
For this test I wanted to be able to see what the camera could do with a given subject looking at it from UV, through the visible to the IR, in effect across the full range of sensitivity. To do this I used a light source which produced light across that range (Bowens GM500 flash units with quartz tubes), a Zeiss UV Sonnar 105mm f4.3 lens, which I have previously mentioned here, and is a very special lens made of quartz and calcium fluoride elements rather than glass, making it suitable for deep UV work. Here’s how the lens looks mounted on the camera.
For a subject, a bunch of flowers from my local florist – Tangerine and Green, Englefield Green. I also included a Colourchecker chart, although as some of you may notice it looks a little odd. I cut the bottom row of white, grey and black tiles off to run in a spectrometer a while back, so it is now just the coloured tiles. Oh, the challenges of science. The images below firstly show the flowers in the visible spectrum (taken on a camera phone) and then in black and white from the IQ4 Achromatic from the UV through to the IR using a range of different filters which are mentioned below each image.
With the images above, they have been reduced hugely in size from the original resolution for sharing on here. Also the flash settings and ISO needed changing for some of the images, especially towards the ends of the wavelength range.
Overall, the flowers demonstrated a huge change their appearance in the different wavebands. The digital collodion image (with the Schott BG18 + BG25 filters) is an interesting one. I’ve written about that filter stack before, here, and it is interesting to see how different the flowers look with it compared to both UV and the broader visible light images. As expected the flowers look fairly similar with the various different IR filters.
Again, very impressed with the Phase One IQ4 Achromatic. It was certainly able to capture images from the UV through the visible and into the IR, when used in combination with suitable lens, filters and light source. I will have more to come from my evaluation of the camera, when I look at its use for landscape photography in the visible and IR regions. Thanks for reading, and if you’d like to know more about this or other aspects of my work, you can reach me here.
I was recently fortunate enough to be able to spend a few days evaluating a Phase One IQ4 Achromatic camera back, courtesy of Teamwork Digital Ltd here in the UK. This back really interested me as it is a medium format (53.4mm x 40.0mm) back side illuminated sensor with no Bayer filter layer. Given my work imaging outside of the visible spectrum, this had a lot of potential, so I wanted to see what it could do. I wont go through all specs of the camera here, but this is prestige piece of imaging equipment and for more information about it head to the Phase One website.
Working with a sensor this large does present some challenges if you are used to cameras will full frame or APS-C sensors, chiefly that of the image circle produced by the lens you use. I have a few Hasselblad lenses designed for the old 6×6 cameras from my film camera days, but more on that in a future post. For the work I’ll be showing here I actually used an El-Nikkor 80mm f5.6 enlarger lens (old version with the chrome body), and this is shown mounted on the camera below.
Mounting the enlarger lens was relatively straightforward – Teamwork provided me with an adapter to go from the Phase One XF body to Hasselblad, and then I had a Hasselblad to M39 adapter setup from Zörk which has a ball joint (for tilt work) and a helicoid (for focusing). A setup like this makes macro imaging nice and simple, and this older version of the El-Nikkor 80mm has good UV transmission as well.
Subject wise, I used some lovely flowers from my local florist – Tangerine and Green, Englefield Green – as I knew I could get some which would have nice UV signatures. Lighting was done using a single Bowens GM500 studio flash head with a quartz flash tube which I had custom made. Images were taken in the UV using a Baader U filter, and in visible light using a filter stack of Schott S8621 1.5mm and a 420nm longpass filter.
Firstly, a sunflower in UV.
The UV sunflower image shows the expected behaviour of the dark centre to the flower, and the dark inner parts of the petals. The image above is the full frame shot captured by the camera – no cropping. Obviously the image above has been reduced in resolution for sharing here. As an example of how much information there is in one of the images produced by this IQ4 back, take a look at the two images below.
The images above shows the sunflower in UV again, this time with a red square that in the original full size photo would be 1000 x 1000 pixels. A crop showing this area in the original image resolution is shown below.
The amount of information contained within one of the images that the IQ4 back can produce is staggering. And keep in mind, I’m using an enlarger lens here, not one of the new lenses built for these cameras, so I doubt the resolution in my images is as good as what can be obtained.
The imaging setup for the flower close-ups is shown below along with a second flower that was imaged. I’ll be honest I’m not sure what this flower is (could be a Dahlia, however that is a bit of a guess), but it looked nice, and I thought it would make an interesting subject.
This is how the flower looked when imaged in visible light.
And now the same flower under UV light.
This flower looked very different under UV – it goes from being very pale in the visible spectrum to absorbing most of the UV light and looking almost black, apart from right in the centre.
Overall I was really impressed with the Phase One IQ4 Achromatic. There’s no doubting it is a premium product and it does come with a premium price tag, but the images it is capable of producing are certainly up there in terms of quality. Thanks again to the guys at Teamwork Digital Ltd for making this work possible, and to you for reading this. I will be sharing more images taken with this camera in future posts, including some Infrared ones, so check back in and see what else there is. If you’d like to know more about this or any other aspect of my work, I can be reached here.
Those that have read my posts before will know that I have a bit of a thing for UV imaging, and that I have been been building a UV microscope capable of imaging down to and below 300 nm. While doing so I’m always on the look out for second hand equipment which can either help with the work, or is of historical value to the whole area of UV microscopy. Today’s post provides some historical context to the development of UV microscopy and shows a couple of the early objectives designed for that job – the Zeiss Monochromats.
The key reason for the development of UV microscopy was the goal of improving resolution – as the wavelength decreases the maximum theoretical resolution that can be reached improves. It made sense therefore to move from visible to UV to see what could be achieved. This increase in resolving power is quite marked, as was noted in ‘Practical Photo-Micrography’ by JE Barnard, 1911, “The objectives made for direct use with ultra-violet light are called ‘monochromats’, and the wave-length for which these are corrected is 275 µµ [275 nm]. The N.A. of the highest power lens in the series is 1.25; but by the virtue of the shortness of the wave-length of the light, the resolving-power is equal to an objective used with white light of 2.5 N.A. – an objective which of course at the present time it has not been possible to produce.”. And nor is it today over a 100 years later. As a side note, the textbook used 275 µµ for the wavelength, and I added the [275 nm] as nm is more commonly used today. As a good friend reminded me, the way the book showed it is a little odd and a more correct way of showing it would be 275 mµ, or as is given on the objectives below 0.275 µ. Not sure why it was shown like this, but wanted to clarify in case of confusion.
What are these ‘monochromats’? Zeiss produced a range of them with focusing distances from 16 mm down to 1.7 mm, and these were summarized in a table in ‘Die Wissenschaftliche und Angewandte Photographie’ by Kurt Michel, 1957;
The Monochromat objectives were made from quartz lens elements, and were designed to be used dry (trockensystem) or with glycerin immersion. Glycerin rather than oil is used for these UV objectives as it remains transparent even down to 250 nm unlike the oils. They were also designed for use at specific wavelengths in the UV – 0,257 µ [257 nm] and 0,275 µ [275 nm]. These wavelengths were chosen as they are the strong emission lines for mercury (in mercury xenon lamps) and cadmium (from a cadmium arc source) respectively. This does not mean that they can be used at other wavelengths, but it is likely that image quality would suffer as a result. The 16 mm Monochromat might be a later edition to the range, as although it is mentioned in Michel’s book of 1957, it did not appear in Barnard’s text of 1911 (which only mentioned 6 mm, 2.5 mm and 1.7 mm objectives).
Enough waffle from me, what do these look like? I’ve been fortunate enough to find two of these objectives a 16 mm one and a 1.7 mm one. The 1.7 mm one was for sale on a ‘well known internet auction site’ and has come over to the UK from Ukraine, the 16mm one came from Germany where it became available for ‘the cost of the shipping to the UK’ as it was of no use to the original owner. It’s nice when that happens.
Here’s the 1.7 mm one, with its original objective keeper.
And the 16 mm one;
Both of these were designed to be used with the Cadmium line at 275 nm and both are RMS threaded. In theory they should be for a 160 mm tube length microscope, which is what I have, so I will certainly be trying these out. However it wont be at 275 nm – the lowest I can go with my current setup is 313 nm, and I have no plans on building a cadmium arc lamp (‘elf and safety and all that). I’m expecting relatively soft images, especially when compared with the Ultrafluar lenses, but you never know. Slightly worryingly, JA Needham in ‘The Practical Use of the Microscope’, 1958, said that “The quartz monochromats were corrected for one wavelength (275 mµ) [275 nm] and could not be employed with other wavelengths in the ultra-violet.”. However we shall see after some testing. I’ll also run these through my lens transmission measurement setup to get their transmission between 280 nm and 420 nm. While I’m not expecting any surprises with them (as they should be of all quartz construction), I like to check all new lenses that come in.
The history of imaging and microscopy is fascinating, especially when you dig into the details of how these amazing scientists and engineers tried to push the boundaries of what was achievable at the time. UV microscopy while being a technique that developed over a 100 years ago, is seldom used today as other ways of improving resolution have since come along. However it has huge potential in the field of imaging sunscreens, so is an area I will be continuing to explore and develop. Thanks for reading, and if you want to know more about this or other aspects of my work, I can be reached here.
When I’ve had a good experience with a supplier I like to try and mention them on my page, if nothing else as a bit of thank you. This brings me to Tangsinuo, a Chinese optical filter supplier. I recently found myself in need of some UV transmitting filters in larger sizes than I would normally be able to buy from Schott or other conventional suppliers. Big filters tend to command big (really) price tags even when they are available and this ruled out the more conventional suppliers for this work. ZWB glass is often seen advertised on ebay and other sites as being ‘equivalent’ UG1, UG11 and others, although some of the transmission graphs that come with the adverts have left me wondering how good they actually are.
One supplier – Tangsinuo – has been mentioned a few times on the UV photography forum which I’m member of, so I reached out to them to see if they could do large filters (200mm square) using the glasses I was interested in and was pleasantly surprised to find out that they could, and that they were very cost effective. In the end I placed an order for some 200mm square ZWB1 and ZWB3 glass, some 77mm diameter mounted filters in ZWB1 and 3, some 52mm filters in ZWB3 at different thicknesses, and also a load of fused silica 1mm thick microscope slides (75mm x 25mm) which are normally quite expensive items. All in all the price as extremely good even when I paid for expedited delivery – just over a week from China to the UK using tracked delivery.
So, how did they look? Transmission spectra of the filters, and the filter thicknesses, are given below (as measured using my Ocean Insight FX and STS spectrometers).
ZWB1 is said to be equivalent to Schott UG11, and it looks similar, although does leak a bit more IR than the Schott for an equivalent thickness. ZWB3 is said to be equivalent to Schott UG5, and again it looks similar. Overall, very impressive especially given the price.
I’ve not shared the transmission spectra of the fused silica microscope slides here, as well they are pretty much a flat line from 250nm to 1100nm and no where near as interesting as the filters.
My filters had a good surface finish (no surface striations which can sometimes be seen with some of these Chinese filters – I have a couple myself) and were chamfered at the edges.
So, thank you Tangsinuo. It should also be noted that they are open to working with customers on custom sizes and thicknesses too which is great to hear. Great prices and professional customer service are nice to experience, so please keep it up. If you’d like to know more about this or any other aspect of my work, you can reach me here.